{"id":2427,"date":"2013-07-17T12:18:00","date_gmt":"2013-07-17T10:18:00","guid":{"rendered":"https:\/\/jakobssonlab.iob.uu.se\/?p=2427"},"modified":"2024-11-26T12:37:01","modified_gmt":"2024-11-26T11:37:01","slug":"2427","status":"publish","type":"post","link":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/2013\/07\/17\/2427\/","title":{"rendered":"Sex identification of ancient remains"},"content":{"rendered":"\n<p><strong>July 17, 2013<\/strong>. Our paper by <a href=\"https:\/\/doi.org\/10.1016\/j.jas.2013.07.004\" target=\"_blank\" rel=\"noreferrer noopener\">Skoglund et al. (2013) on &#8220;Accurate sex identification of ancient human remains using DNA shotgun sequencing<\/a>&#8221; was published by the Journal of Archaeological Science and highlighted by <a lang=\"none\" href=\"http:\/\/www.nature.com\/nature\/journal\/v500\/n7460\/full\/500009a.html\"><em>Nature<\/em><\/a>.<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-6c531013 wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image size-full is-resized\"><img decoding=\"async\" src=\"https:\/\/jakobssonlab.iob.uu.se\/wp-content\/uploads\/2024\/11\/Journal-of-archaeosciences2013.jpg\" alt=\"\" class=\"wp-image-2428\" style=\"width:300px\"\/><\/figure>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-fe9cc265 wp-block-group-is-layout-flex\">\n<h2 class=\"wp-block-heading\">Highlights<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u2022We present a simple sex identification method using low-coverage DNA sequencing.<\/li>\n\n\n\n<li>\u2022The approach is validated using a panel of 30 modern and ancient individuals.<\/li>\n\n\n\n<li>\u2022The method can be robust to modern-day contamination by using degradation patterns.<\/li>\n\n\n\n<li>\u2022The study illustrates the risk of misidentification of sex using morphological approaches.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n\n\n<p>Accurate identification of the biological sex of ancient remains is vital for critically testing hypotheses about social structure in prehistoric societies. However, morphological methods are imprecise for juvenile individuals and fragmentary remains, and molecular methods that rely on particular sex-specific marker loci such as the amelogenin gene suffer from allelic dropout and sensitivity to modern contamination. Analyzing shotgun sequencing data from 14 present-day humans of known biological sex and 16 ancient individuals from a time span of 100 to \u223c70,000 years ago, we show that even relatively sparse shotgun sequencing (about 100,000 human sequences) can be used to reliably identify chromosomal sex simply by considering the ratio of sequences aligning to the X and Y chromosomes, and highlight two examples where the genetic assignments indicate morphological misassignment. Furthermore, we show that accurate sex identification of highly degraded remains can be performed in the presence of substantial amounts of present-day contamination by utilizing the signature of cytosine deamination, a characteristic feature of ancient DNA.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>July 17, 2013. Our paper by Skoglund et al. (2013) on &#8220;Accurate sex identification of ancient human remains using DNA [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":2428,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12,18,11],"tags":[56,78,46,36,77,79],"class_list":["post-2427","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news","category-publications","category-research","tag-ancient-dna","tag-dna-analysis","tag-genomics","tag-human-past","tag-sex-identification","tag-shotgun-sequencing"],"_links":{"self":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/2427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/comments?post=2427"}],"version-history":[{"count":2,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/2427\/revisions"}],"predecessor-version":[{"id":2432,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/2427\/revisions\/2432"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/media\/2428"}],"wp:attachment":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/media?parent=2427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/categories?post=2427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/tags?post=2427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}