{"id":3236,"date":"2026-01-19T13:00:01","date_gmt":"2026-01-19T12:00:01","guid":{"rendered":"https:\/\/jakobssonlab.iob.uu.se\/?p=3236"},"modified":"2026-05-12T13:11:39","modified_gmt":"2026-05-12T11:11:39","slug":"tweap-prepress20260116","status":"publish","type":"post","link":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/2026\/01\/19\/tweap-prepress20260116\/","title":{"rendered":"Tweap protocol\u2014Lets Tweap again: Economic and SNP retrieval optimisation for target enrichment of ancient DNA"},"content":{"rendered":"\n

The protocol (closely related to the preprint described below) has been updated and published in April 2026 (Open Access): Saupe, T., Tan, E. J., G\u00f3mez, M. O., Bernhardsson, C., & Jakobsson, M. (2026). Tweap protocol for target enrichment via in-solution hybridisation using Twist Bioscience Panels. protocols.io. DOI: https:\/\/www.protocols.io\/view\/tweap-protocol-for-target-enrichment-via-in-soluti-5qpvoknbxl4o\/v1<\/a><\/p>\n\n\n\n

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An updated preprint from the Jakobsson Lab: Saupe, T., Omar G\u00f3mez, M., Tan, E. J., Kabral, H., Scheib, C. L., Bernhardsson, C., & Jakobsson, M. (2026). Lets Tweap again: Economic and SNP retrieval optimisation for target enrichment of ancient DNA. bioRxiv<\/em>, 2026-01. https:\/\/doi.org\/10.64898\/2026.01.15.699081<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n

Abstract<\/h2>\n\n\n\n

Advancements in ancient DNA (aDNA) research have enabled the recovery of individual human genomes through target enrichment approaches. Despite the decreasing cost of next-generation sequencing, enrichment methods targeting single-nucleotide polymorphisms (SNPs) compiled in RNA\/DNA hybrid panels remain costly and require further authentic optimisations. Here, we present the Tweap (Twist cheap) protocol as a cost-effective alternative to the commercialised Twist protocol for target enrichment via in-solution hybridisation of ancient human DNA by replacing the streptavidin-coated superparamagnetic beads for binding the hybridised targeted DNA. We evaluated the protocol on 14 double-stranded DNA libraries from ancient human individuals originating from a hot and humid climate zone known to accelerate DNA degradation. The selected individuals reflected similar preservation levels and a broad range of endogenous human DNA content (0.79-41.69%). We optimised and simplified both the Twist and Tweap protocols for target enrichment, particularly for libraries with ultra-low library complexity, making it reproducible in any life sciences laboratory. The modified Tweap protocol not only reduced overall reagent costs and consumption, contributing to more climate-conscious research, but also recovered up to 7% more unique SNPs when applying multi-pooling of libraries from the same individual. Our findings demonstrate that the Tweap protocol is a viable, efficient, and scalable alternative for SNP capture in ancient DNA (aDNA) studies, supporting broader accessibility and sustainability in ancient DNA research.<\/p>\n","protected":false},"excerpt":{"rendered":"

The protocol (closely related to the preprint described below) has been updated and published in April 2026 (Open Access): Saupe, […]<\/p>\n","protected":false},"author":2,"featured_media":3309,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18,11],"tags":[56,113,223,224],"class_list":["post-3236","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications","category-research","tag-ancient-dna","tag-methods","tag-snp-retrieval","tag-tweap"],"_links":{"self":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/3236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/comments?post=3236"}],"version-history":[{"count":3,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/3236\/revisions"}],"predecessor-version":[{"id":3310,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/posts\/3236\/revisions\/3310"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/media\/3309"}],"wp:attachment":[{"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/media?parent=3236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/categories?post=3236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/jakobssonlab.iob.uu.se\/index.php\/wp-json\/wp\/v2\/tags?post=3236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}